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F12培养基 Gibco试剂

F12培养基 Gibco试剂

简要描述:F12培养基 Gibco试剂
英文名称:Hams F-12 Nutrient Mix, powder
规格:10 x 1 L
性状:干粉培养基
货号:21700-075

产品型号: 21700-075

所属分类:Gibco干粉培养基

更新时间:2015-12-30

厂商性质:经销商

详情先容

F12培养基 Gibco试剂 原装正品,质量保证,现货供应 450元/盒


英文名称:Ham's F-12 Nutrient Mix, powder
规格:10 x 1 L
性状:干粉培养基
货号:21700-075

F12培养基 Gibco试剂 产品描述:

描述

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco? F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

WithWithout
• L-glutamine• Sodium Bicarbonate
• Phenol Red 


The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
For supply chain continuity, Life Technologies manufactures Gibco? F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers. In addition, the New York facility has ISO 9001 certification.

Gibco? F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco? cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

产品规格:

Form:Powder
Glutamine:L-Glutamine
HEPES Buffer:No HEPES
Product Size:10 x 1 L
Phenol Red Indicator:Phenol Red
Sodium Pyruvate Additive:Sodium Pyruvate
Shipping Condition:Room Temperature

培养基配方

21700 - Ham's F-12, powder

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