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N2615 RNasin Plus Promega试剂

N2615 RNasin Plus Promega试剂
N2615 RNasin Plus Promega试剂

N2615 RNasin Plus Promega试剂
规格:10000u
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产品先容

N2615 RNasin Plus Promega试剂?

原装正品,质量保证,现货供应

规格:10000u

N2615 RNasin Plus Promega试剂? 产品描述:

组分:

 

RNasin® Plus RNase Inhibitor【子货号:N261B,包装:1 x 10,000u,,运保温度:–20°C】

 

 

描述:

 

说明

 

RNasin® Plus RNase Inhibitor (RNasin® RNA 酶抑制剂) 是一种作为可溶性蛋白在E. coli 中表达的重组哺乳动物RNase 抑制剂,可以利用离子交换和疏水层析相结合的方法纯化获得。该蛋白能够抑制真核RNase( 如RNase A 和RNase B) 活性,与人胎盘RNase 抑制剂相似。RNasin®Plus RNase Inhibitor 经过RT-PCR 检验,能与AMV、M-MLV 和ImProm-II™ Reverse Transcriptases 或Taq 及Tfl DNA Polymerases 兼容。RNasin® Plus RNase Inhibitor 在TaqMan®检测中发现也能和定量、实时RT-PCR 兼容。

与人源的这种蛋白相比,该抑制剂具有更高的抗氧化活性。研究表明来源于人的该蛋白的两个半胱氨酸对氧化非常敏感,并形成二硫键封闭了抑制剂的活性位点。由于天然氨基酸的多样性,RNasin® Plus RNase Inhibitor 不能形成封闭位点的二硫键。此外,还发现这种重组蛋白具有以前从未认识的特性,即在50℃以上能持续抑制RNase。将RNasin® Plus RNase Inhibitor 和RNase 的溶液加热再冷却,不会造成RNase 活性的恢复——即使溶液温度超过RNasin®Plus 蛋白的变性温度。因此,RNasin® Plus RNase Inhibitor 能够在加热前、加热中及加热后保护RNA,即使在RT-PCR 中*链DNA合成温度下亦如此。将溶液置于70℃,15 分钟,没有观察到RNase的活性恢复。

 

特点 

. 增强的抗氧化能力:由于天然氨基酸的多样性,RNasin® Plus RNase Inhibitor 不能形成人源蛋白在氧化条件下会形成的封闭
活性位点的二硫化物。

• 高纯度:RNasin® Plus RNase Inhibitor 是E. coli 表达的可溶蛋白,利用离子交换和疏水相互作用层析相结合的方法很容易纯化获得。不需要直接亲和层析。这种方法可产生纯度>90% 的抑制剂蛋白,无 E. coli RNase 残留。

• 与RT-PCR 系统兼容:RNasin® Plus RNase Inhibitor 已被证明可与Access and Access Quick™ RT-PCR Systems、ImPromII™ Reverse Transcription System 和Reverse Transcription System 兼容。同样也被证明可与基于TaqMan® 的RT-PCR systems 兼容。

• 在RNA 模板变性过程中保护RNA:即使在70℃加热RNasin® Plus RNase Inhibitor 和RNase 的混合物15 分钟,也不会导致RNA 酶的活性复苏。许多RT-PCR 操作方法需要在RT反应前将RNA 模板在RT 引物存在时变性( 例如65-70℃,5-10 分钟) 以获得zui大灵敏度。现在您可以在这一步骤中加入RNasin® PlusRNase Inhibitor。

• 可保护较高温度下的RT 反应:可在RT 反应过程中加入RNasin® Plus RNase Inhibitor,与ImProm-II™ 和AMV Reverse Transcriptases 等一起在50℃以上进行反应。在高温下可能存在的RNase 会失活。

• 可定制或批量订购:从以下获得更多关于该产品的定制选项:www.promega。。com/myway/。

 

 

 

原厂资料:

 

RNasin® Plus RNase Inhibitor is a recombinant mammalian RNase inhibitor that is expressed as a soluble protein in E. coli, allowing easy purification through a combination of ion exchange and hydrophobic interaction chromatography. The protein is capable of inhibiting eukaryotic RNases (e.g., RNase A and RNase B) similarly to human placental RNase inhibitor. RNasin® Plus RNase Inhibitor is tested in RT-PCR and is compatible with enzymes such as AMV, M-MLV and ImProm-II™ Reverse Transcriptases or Taq and Tfl DNA Polymerases. RNasin® Plus RNase Inhibitor also is tested and compatible with quantitative, real-time RT-PCR in a TaqMan® assay.

The inhibitor offers increased resistance to oxidation over the human version of the protein. Two cysteines in the human protein have been identified as especially sensitive to oxidation and react by forming a disulfide bond that can block the active site of the inhibitor. RNasin® Plus, through natural amino acid diversity, lacks the ability to form this site-blocking disulfide. In addition, the new protein has characteristics never before realized, including continued inhibition of RNases above 50°C. Heating solutions of RNasin® Plus and RNase followed by cooling does not result in the reappearance of RNase activity—even when the solution is heated above the denaturation temperature of the RNasin® Plus protein alone. This allows RNasin® Plus to protect RNA species prior to, during and after heating, even at temperatures normally used during first-strand DNA synthesis in RT-PCR. We have taken solutions up to 70°C for 15 minutes and did not see RNase reactivation.

Features - Benefits

  • Improved Resistance to Oxidation: Due to natural amino acid diversity, RNasin® Plus lacks the capability to form the active site-blocking disulfide bond that can form in the human protein under oxidative conditions.
  • Improved Purification: RNasin® Plus is expressed by E. coli as a soluble protein, allowing easy purification by a combination of ion exchange and hydrophobic interaction chromatography. No direct affinity chromatography required. The new process yields a >90% pure protein with no E. coli RNase carryover.
  • Proven Compatibility with RT-PCR Systems: RNasin® Plus has proven compatible with the Access and AccessQuick™ RT-PCR Systems, ImProm-II™ Reverse Transcription System and the Reverse Transcription System. Also proven compatible with TaqMan®-based RT-PCR Systems.
  • Protection During RNA Template Denaturation: Heating mixtures of RNasin® Plus and RNase does not lead to reactivation of the RNase at temperatures even as high as 70°C for 15 minutes. Many RT-PCR protocols call for RNA template denaturation (e.g., 65–70°C for 5–10 minutes) in the presence of the RT primers prior to full RT reaction assembly for maximum sensitivity. You can now include RNasin® Plus at this step.
  • Protection During Higher Temperature RT Reactions: Add RNasin® Plus during RT reaction assembly and take the reaction to temperatures above 50°C with enzymes like the ImProm-II™ and AMV Reverse Transcriptases. RNases that may be present will not be reactivated at the higher temperature.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。com/custom/

Applications

  • RT-PCR.
  • Quantitative, real-time RT-PCR.
  • In vitro transcription.
  • In vitro translation.
  • Coupled transcription/translation.
  • Useful in any applications where eukaryotic RNase contamination is a potential problem.
  • Protection of mRNA in cDNA synthesis reactions.

References

  1. Kim, B.M. et al. (1999) Protein Sci. 8, 430–4.

? 相关产品列表:

Promega 系列
序号货号  名称 规格
1M1801  T4连接酶         100U 
2M1804      T4连接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         

产品相关关键字: Promega试剂

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Promega普洛麦格C1101 Oligo(dT)15 Primer 20ug 现货  GoTaq DNA Polymerase 100u 5u/μl Promega普洛麦格M3001现货  GoTaq DNA Polymerase 500u 5u/μl Promega普洛麦格M3005现货 
M4103 T4 Polynucleotide Kinase Promega试剂  M4101 T4 Polynucleotide Kinase Promega试剂  M4215 T4 DNA Polymerase Promega试剂 
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